cimH [2019-08-12 13:26:50]

transporter for citrate (proton symport)

Locus: BSU_38770

Isoelectric point: 6.55

Molecular weight: 48.18 kDa

Protein length: 450 aa

Gene length: 1353 bp

Function: citrate uptake

Product: transporter for citrate (proton symport)

Essential: no

Synonyms: yxkJ

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Transporter for organic acids
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,979,753 → 3,981,105

The protein

Catalyzed reaction/ biological activity

• uptake of citrate (symport with protons) PubMed

Protein family

• sodium:citrate (SCF) symporter family (together with MaeN, according to UniProt)

Paralogous protein(s)

• MaeN

Structure

• 5X9R (from Klebsiella pneumoniae, 34% identity) PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cimH
  Description: PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • repressed by glucose (CcpA) PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B744 (yxkJ::erm), available at the NBRP B. subtilis, Japan
• BKE38770 (ΔcimH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• BKK38770 (ΔcimH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT, downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC

References

Reviews

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Original publications

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